Prof. M. Memo

Dept. of Molecular and Translational Medicine

University of Brescia, Italy

Forty-eight, 4-6 month-aged, male mice were divided into 6 different experimental groups (8 mice for each group). Mice had free access to food. To evaluate food intake, the amount of food available for each cage was weighted every 24 hours.

Food consumption during the first 3-Day stabilization period was constant with no difference in the experimental groups. The first gavage treatment at Day 4 resulted in a stress-induced response that was common to all the experimental groups. This effect caused a short· lasting decrease in food consumption that disappeared in all groups except in the Rimonabant and our final formula group.

When comparing food consumption at Day 6 (3-day after cannabinoid treatment) to that of Day 3

(3-day after stabilization period) we found a significant decrease in both Rimonabant and our formula.  

Surprisingly, the results clearly demonstrate that our final formulation possess the unexpected property to reduce food consumption and body weight in mice after 3-day treatment. With the dosage used and at the time (duration) of treatment and evaluation, all the other combinations that lacked the final cannabinoid were unable to induce such effects.

These data highlight the concept that mixing different cannabinoids may result in a novel compound with unexpected pharmacological properties far from the simple algebraic view of agonist/antagonist action at receptor level. ‘We found that only the final cannabinoid formula induces a reduction in food consumption, which is fundamental for weight loss.

The individual compounds THCV and CBx themselves have no effect on eating behavior and weight loss.